CAMBRIDGE, Mass. — “There’s a lot of YouTube videos on this,” said Avery Normandin on a gray Sunday morning in late October. “But they’re pretty” — he used an unprintable word — “so I’ll try to do a better job.” He is a grad student in the MIT Media Lab’s Sculpting Evolution group, where some members have been trying to genetically engineer mice to keep them from harboring tick-borne illnesses, and have been discussing the release of these rodents with residents of Lyme-disease-plagued islands like Nantucket and Martha’s Vineyard.
Now, though, Normandin was surrounded by an entirely different kind of laypeople. These folks — or at least some of them — were hoping to try gene editing for themselves. There was an IT consultant from Hong Kong. There was a former teacher who’d joined a community lab in Southern California. There was a high schooler from Thailand. There was a sculptor from Baltimore, who’d recently started exploring biomaterials — “I’ve been sewing dried fish stomachs, experimenting with hydrogel expansion and growing mycelium in silicone molds,” she explained — and was thinking of incorporating living creatures into her art.
They’d all flocked to the Massachusetts Institute of Technology for a three-day summit of biohackers from around the world, an event with the aim of democratizing science, taking technology that’s often hidden in keycard-access labs at Harvard or Stanford and bringing it to the people. It’s the kind of conference where someone might stop you in the street and insist you take a foldable microscope and a handful of paper slides.
Sure, there were panels you might find at more academic get-togethers — about biosafety and scientific publishing and storytelling in biotech — and there were plenty of Ph.D.s in attendance. But there were also sessions with names like “Let’s swab,” “Hacking your way to affordable healthcare technology that reaches the last mile,” and “Sequencing genomes of the entire planet and open-sourcing them, not if but when.”
This breakout session had begun with the ringing of a cowbell, and a gaggle of participants walking across the street to a biology building for a workshop called “Hands-on CRISPR.” It was in the right place for tinkering — a windowless wet lab, with receptacles for biowaste — but it turned out that the only CRISPR the participants would be handling was of the marker-and-whiteboard variety.
They’d all heard and read plenty about this revolutionary genome-editing technology, and knew that it was, as cutting edge lab tools go, cheap and easy to work with. Now Normandin was going to give the beginners among them an insider’s look into how it actually works. He’d envisioned leading the group through an experiment on E. coli, but as he explained later, “I never want researchers — at any level — to be ‘driving blind.’ Context is crucial!”
Perched on a stool in white socks and sandals and a baseball cap, he began to draw part of a bacterial genome, with green boxes separated by lines, a bit like the buoys on a rope that might prevent campers from swimming outside the shallow end of a lake. “These repeat sequences are telling us something,” he said. “They’re regularly interspersed …” He stopped. “Interspaced? Spersed? Spaced?”
He went on, explaining that some of this sequence inside the bacteria was identical to the genetic material of an invader, like a bacteriophage — a virus that can attack bacteria. He drew a phage, which resembled a spider from outer space. He said some bacteria learned how to recognize and deal with such parasites by taking some of the viral genetic sequence into their own. Then, when they identified the same chain in a later attacker, they could produce an enzyme to chop it up.
“That’s a gross, globular protein,” he said, drawing a blob to represent the bacteria’s enzymatic weapon.
“I’m kind of lost,” said the former teacher.
“Come closer,” Normandin replied. “You’re going to draw it for us. Here’s a marker.”
Then, he explained that there was a tiny piece of the viral genome called a PAM that shows the bacteria’s anti-virus task force where to focus. “It’s a giant ‘Kick me’ sticker!” he said.
“What does PAM stand for?” someone asked.
“Protospacer adjacent motif,” said the sculptor, looking at her phone. “I Googled that.”
A discussion ensued about how a bacterium would have the chance to evolve defenses against a viral attacker. Wouldn’t the first attack be successful if you don’t already have defenses? Normandin explained that a phage with faulty machinery might open up that window of possibility.
“I’m still confused about what a phage is,” said the sculptor.
Normandin erased the whiteboard with his hands, marker staining his fingers blue and green. He started drawing some genomes from scratch. “Gosh, this is the hardest thing I’ve ever done,” he said, smiling.
Concept of cutting out disease was mentioned by Shakespeare, Macbeth asking the physician to treat lady Macbeth: 1606: Canst thou not minister to a mind diseased, pluck from the memory a rooted sorrow, raze out the written troubles of the brain, and with some sweet oblivious antidote cleanse the stuffed bosom of that perilous stuff which weighs upon her heart.Gene editing, looks easier, but the sad part is that very clever about tinkering with the gene DNA, causing perturbation are only test half of what MUST BE DONE. Givenchy designing dresses for Audrey Hepburn took equally good care of the suitable top cover coat as well. DISEASED persons’ Cell Gene DNA editing by CRISPRCas9, CRISPR-Pf1 or search-and-replace are only half testing for what are called unwanted insertion errors that in time might and some times do cause cancer, leaukaemia deaths! Edited gene cells are not test fully wrt to specific receptor before transplantation, and after therapy, regenerated cells (supposedly perfect) in treated patients. The patient will need to be followed for 10 to 15 years.
The Nobel committees usually wait in such cases, hope they will. Peyton Rous was not awarded the Nobel prize until he was well advanced in his years.
I wish one could persuade the ‘aspirants’ and others showing total lack of awareness to test thoroughly inside, outside parts, receptors of cells. If they dot know, just ask what a Cambridge undergraduate or at Harvard, Yale, Caltech can tell them. Blind spots need light thrown on them for safety, health and welfare.
By harnessing this technique, scientists could, say, genetically modify mosquitoes to only produce male offspring — and then use a gene drive to push that trait through an entire population. Over time, the population would go extinct. “Or you could just add a gene making them resistant to the malaria parasite, preventing its transmission to humans,” Vox’s Dylan Matthews explains in his story on CRISPR gene drives for malaria .
Encouraging nonscientists who struggle to grasp the concept to practice gene splicing in their garages for frivolous reasons – what could possibly go wrong?
“There was a sculptor from Baltimore, who’d recently started exploring biomaterials — “I’ve been sewing dried fish stomachs, experimenting with hydrogel expansion and growing mycelium in silicone molds,” she explained — and was thinking of incorporating living creatures into her art.”
It’s a great time to be alive
democratizing science, or in other words, power to ignorance and dumbness
Ha! While I loved the tongue-in-cheek…I mean isn’t context the point? They are educating laymen…not giving them license (the internet will do that quite well…thank you!:). Best to educate properly…or attempt to (while admitting the artist with the fish quote was simply gobsmacking! )
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